Sujitha Asadhi

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8019272524

Sri Padmavati Mahila Visvavidyalayam, India

University of Milan

Department of Agricultural and Environmental Sciences-production, Territory, Agroenergy, Italy

Development and assessment of rapid and cost-effective DNA extraction protocols suitable for marker-assisted selection in stone fruit species. The apricot tree (Prunus armeniaca L.) is widely distributed stone fruit species in worldwide. In the main production areas, apricot culture is nowadays threatened by sharka, a disease caused by Plum pox virus (PPV), which belongs to the Potyvirus genus within the family Potyviridae. In order to demonstrate the resistant or susceptibility behavior of Prunus armeniaca L genotypes against Plum pox virus through marker assisted selection. Marker assisted selection (MAS) permits the selection of plants carrying genes that control interesting agronomic traits through the screening of associated markers; this allows the breeders to retain only germplasm conferring desirable traits from an early stage. Generally MAS involves the screening of hundreds or thousands of seedlings and thus the main challenge faced is the development of a pipeline that starts with a “lab friendly” an unambiguous seedling disposition in the greenhouse, allows the rapid collection of samples, avoiding cross contamination, implements a fast, cost-effective, high-throughput method of DNA extraction to be used for a small number of PCRs, and utilizes robust, reliable, and easy to interpret molecular markers.