Articles
| Name |
Rajesh Kumar |
| Year of selection | 2016? |
| Cohort | 2 |
|
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|
| Phone number |
8809435010 |
| Home Institute |
Dr Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar-848125, India |
| Host Institute |
UPM, Spain |
| Lab, Institute, Country | CBGP,UPM-INIA, Madrid, Spain |
| Name of Researcher/Supervisor | Prof. Fernando Garcia-Arenal Rodriguez |
| Duration of working period |
20/10/2016 to 20/12/2016 |
| Title and Brief report of the work (max 300 words) |
Site-directed mutagenesis of L1 gene Introduction Tobamo viruses are RNA viruses having positive-sense, single-stranded RNA genome, encapsulated into rigid, rod-shaped particles. Tobamo viruses are contact transmitted and can be classified into pathotypes according to their ability to overcome host resistance. Capsicum species has been characterized to express a resistant protein (encoded by L gene) in response to tobamo virus infection. The resistance against different tobamo viral pathotypes (P0 to P1,2,3) is conferred by four different L gene alleles (L1 to L4) . ➢ Objective: ➢ To convert L1 to thermo resistant L1a allele, four amino acids were selected for Site-directed mutagenesis. Materials and methods: Substitution mutations were introduced by site-directed mutagenesis using the Quick-Change Kit (Stratagene). Plasmid comprising L1 gene (pEAQ-HT-DEST1-L1) was used as a template for generating a mutation in L1 gene. Table :The position of amino acid in L1 gene selected for Site-Directed Mutagenesis. Position of amino acid and corresponding substitution of nucleotide in codon NB domain LRR domain Gene 296 903 977 1260 L1 S (TCT) D (GAC) I (ATT) F (TTC) L1a C (TGT) E (GAA) S (AGT) S (TCC) Results: Plasmid comprising L1 gene (pEAQ-HT-DEST1-L1) was used as a template for the PCR reaction with L1 296 SC F and L1 296 SC R primers. |
| List of publications with impact factor, presentation of the research work in conferences/ seminars /workshops |
Singh,Renu; Singh,Yashi; Xalaxo,Suchit; Singh,A.K; Singh,P.K; Nilanjaya; Singh,N.K; Kumar,Rajesh; Chetia,S.K (2016):From QTL to Variety-Harnessing the benefits of QTLs for drought,flood and salt tolerance in mega varieties of India through a multi-institutional network; Plant Science 242 : 278-287. Impact factor : 4.148 |
| Present position |
Assistant Professor, Department of Plant Breeding and Genetics, RPCAU, Pusa |