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Articles

Name

Avinash Chandra Rai

Year of selection  
Cohort  
e-mail

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Phone number

919305009236

Home Institute  
Host Institute UNIMI, Italy
Lab, Institute, Country

Dipartimento di Scienze Agrarie e Ambientali, UNIMI, Italy

Name of Researcher/Supervisor Prof. G. A. Sacchi
Duration of working period 9 months
Title and Brief report of the work (max 300 words)

Title : Phenotyping of worldwide japonicas rice accessions and bi-parental segregating RILs for identification of salt tolerant genotypes

The proposed research works was based on the identification of salt tolerant genotypes using worldwide japonicas rice varieties. To find out the most tolerant genotype for salt stress condition we were used the worldwide 281 japonica accessions. Along with this we were also focus the possible salt tolerant gene/alleles using the linkage mapping in a population of bi-parental Recombinant Inbred Lines (RILs). To explore the possible genotypes and alleles we were rigorously initiated different phenotypic, physiological and biochemical approaches in glass house and hydroponics conditions. For approaching these experiments we used Infrared thermography, SES, Fv/Fm, Dualex, Plant height along with GWAS analysis for phenotyping of genotypes and RILs lines. Whereas in hydroponics system we used salt tolerant/susceptible core collection lines for their further evaluation using Infrared thermography and ionomic analysis of Na/K partitioning in shoot and leaves using ICP-MS (Inductively coupled plasma mass spectrometry) instrumentation. Stepwise result are summarized below which are mention in the proposed research plan. Thermal images of all the 281 genotypes were acquired using the high throughput infrared (FLIR) camera at late flowering stage. All the images will be analyzed using the infrared thermography software (ReserachIR). After determination of the SSI (stress susceptibility index) analysis of thermal image data, GWAS analysis was performed using the publicly available japonicas rice SNPs databases. To identify possible loci involved in salt tolerance in the considered japonica rice accessions, GWAS analysis was conducted expressing the thermal temperature of the plants phenotypic trait as SSI. The most associated SNPs loci will be further studied for salt stress related traits with available literature search.  Further The RILs developed by CREA using Baldo and Vialone nano as a one of the most salt tolerant and sensitive at F7 stage were evaluated. All the 200 RILs along with their parents were sown in glass house condition (5 plants per pots). At early vegetative stage we were kept two healthy and equal vigour plats per pots for further salt stress exposure and further taking phenotypic data. After achieving desire salt stress condition (4-5 ds m-1) in pots (measuring the conductivity of soils), we were measuring the SES (standard evaluation system) trait data. When first flag leaves were appear we were measures the plant height, flowering time, photosynthesis data (Fv/Fm) and Dualex measurement. The works are still in progress and after taking the whole phenotypic data, they were statistically evaluated and GWAS analysis will be performed for the identification of traits specific promising SNPs and their respective loci on the chromosome. This association mapping study will directly indicate us to our desired traits specific location of our candidate gene with their respective position.  In the parallel experiment all the 281 rice genotypes will re-evaluate in 2nd year for salt stress, measurement of all the phenotypic data were done which was taken in previous year Phenotyping (SES, Plant Height, flowering time, photosynthetic rate (Fv/Fm) and dualex). All genotypes were sown in glass house condition (20 plants per pots). At early vegetative stage we were kept five healthy and equal vigour plants per pot for further salt stress exposure and taking phenotypic data. After exposure of salt stress and achieve desire salt stress condition (4-5 ds m-1) in pots (measuring the conductivity of soils) we were measured the SES data. After first flag leaves appearance we were measure the plant height, photosynthesis rate (Fv/Fm) and Dualex data. For hydroponics experiments, statistical analysis of first year phenotyping of 281 japonicas rice reveals 13 tolerant and 12 sensitive core collection lines. These selected core collection varieties were grown in supported hydroponics (pots containing 50%perlite and 50% sand) nutrient solution. At early age of plantlet (2-3 leaf stage), the salt stress condition was imposed. After 48 hours of salt treatment, infrared thermography analysis of plants had been carried out. Additionally, leaves were separately collected (the oldest to youngest) in order to study the Na and K ion partitioning from oldest to newest leaves. Na and K content have been determined by using the high throughput ICP MS instruments. Level of temperature, distribution and accumulation of sodium ion in different part of plants in this experiments will indicates us to find out the mitigation effect of salt stress and reconfirm the more selective core collection tolerant lines. Further, after analysis of core collection lines in hydroponics in early seedling stage we had concise one promising M203 as tolerant and L201 as susceptible genotypes. For reconfirmation of both the lines again both the genotypes M203 and L201 were sown in complete aerated hydroponics medium. At 2-3 leaf stage again salt stress were imposed along with control. Root, shoot and leaves of the salt treated and controlplantswerecollected. Thermal images and Na/K partitioning using the ICP-MS were reconfirmed the salt tolerant and sensitive lines.

List of publications with impact factor, presentation of the research work in conferences/ seminars /workshops

work was presented at science congress in UDINE, Italy, september 12 2017 and our poster was selected as best poster award

Present position

Post Doctoral Fellow, ARO, Volcani, Israel


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