Somnath Koley

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0049-345-5525092

Orissa University of Agriculture & Technology, India

Martin Luther University, Germany

Pharmaceutical Biology

Terpenoids are synthesized via two distinct pathways i.e. mevalonate pathway (MEV) and non-mevalonate pathway (MEP) . Traditionally monoterpene, diterpene and tetraterpene are mainly synthesized via MEP pathway which occurs in plastid, whereas sesquiterpene and triterpene are mainly produced via MEV pathway which occurs in cytosol . Precursors of both pathways are provided by upper part of central carbon metabolism i.e. glycolysis and pentose phosphate pathway. These pathways synthesize two common isoprenoid intermediates, namely dimethylallyl pyrophosphate (DMAPP) and isopentyl pyrophosphate (IPP) which might have role in cross-talk between these two pathways . Peppermint (Mentha piperita) mainly synthesizes monoterpenes such as Menthone, Menthol, Pulegone, 1,8-Cineole etc. which have significance in pharmacy and also use as anti-bacterial agent. In present study, we firstly optimized light condition of Peppermint shoot tip culture at which plants were grown well enough with very less amount of dilution of labelling which is needed for 13C tracer analysis. In this study, shoot tip culture was grown at four different light conditions i.e. 5, 10, 20 and 30 µmol/m2/s for 15 days and 20 days leaf age. Most amount of 13C enrichment was observed at 5 µmol/m2/s light intensity in 15 days leaf growth stage and with increasing light intensity, dilution of labeling is increasing. 13C tracer analysis was done by using 1-13C, 6-13C and 1,6-13C2 glucose at 5 µmol/m2/s light intensity in 15 days leaf growth stage. From this study, it was observed that pentose phosphate pathway was active at this low light condition. Preliminary observation from 1,6-13C2 glucose experiment showed that there was cross-talk between two terpenoids biosynthesis pathways. To know the contribution of these two pathways for monoterpene synthesis, 13C metabolic flux model has been constructed.

• Biosynthesis of terpenoids in trichomes by S. Koley and B. Junker in 11th Plant Science Student Conference (PSSC) (2015) at Halle (Germany)

• Biosynthesis of terpenoids in Peppermint by S. Koley and B. Junker in Terpnet (2017) at Dalian (China)

Contuning PhD