Ved Prakash

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919141000000

University of East Anglia

RNA Biology Lab, School of Biological Sciences, UEA

Prof. Tamas Dalmay

2/4/2016-02/10/2017

Title of my PhD research is " analysis of tobacco RNA dependent RNA polymerase 1 (RDR1) mediated antiviral response". During the period of BRAVE fellowship at UEA, I generated small RNA libraries from NtRDR1 transgenic N. benthamiana and wild-type plants infected with Tomato leaf curl Gujrat Virus. At the moment further analysis of differentially expressed sequences is in the process. The purpose was to understand the function of RDR1 by analyzing differentially expressed small RNAs. We also generated sRNA libraries from rdr1 mutant Arabidopsis and rdr1 mutant tobacco plant. miRNA 159b was found to be having reduced expression in rdr1 mutant Arabidopsis compared with WT plant which was validated by northern blot analysis.

Prakash V, Devendran R, Chakraborty S. 2017. Overview of plant RNA dependent RNA polymerases in antiviral defense and gene silencing. Indian Journal of Plant Physiology 22: 493-505. https://doi.org/10.1007/s40502-017-0339-3.

Poster was presented in two seminars/conferences: 1. NoCaSS 2017. Title: “Effect of RDR1 mutation on small RNA abundance in Arabidopsis, N. benthamiana and N. tabacum". 2. 86th conference of Society of Biological Chemists. Title: miRNA 159b shows reduced expression in rdr1 mutant Arabidopsis.

At the monent I am in my last year of PhD at School of Life Sciences, Jawaharlal Nehru University, New Delhi, India